Amplification of CT converted DNA and expression analysis. (A) Immunocytochemistry of β-adrenergic receptors, showing strong expression of β1and β3-adrenergic receptors. (B) Amplification of CT converted DNA using bisulfite converted primers for β1, β2 and β3-adrenergic receptors. (C) Expression levels of β1, β2 and β3-adrenergic receptors genes by reverse transcription, in high and normal glucose conditions. GAPDH was used as a loading control while cDNA without Reverse Transcriptase was used as negative control. (D) Graphical presentation of β1, β2 and β3-adrenergic receptors expression of mRNA (E) Expression of β1, β2 and β3-adrenergic receptors at protein level by Western Blotting in high and normal glucose. (F) Graphical presentation of relative expression of β1, β2 and β3-adrenergic receptors genes in retinal endothelial genes.