DNA methylation status of SNORA12 in Mock or Human Rhinovirus (HRV) infected nasal epithelial cells (NECs). A. Schematic of SNORA12 pyrosequencing assay design. In addition to the CpG targeted by probe cg02440370 (#1), a second CpG (#2) was identified within the coding region. Both CpGs were amplified in bisulphite-specific PCR (Bi-PCR). B. Analysis of SNORA12 methylation in both study populations (see Table 1) revealed a significant increase at CpG#1 within the Healthy NECs during infection. No changes were observed at CpG#2 (data not shown). C. Change in CpG#1 methylation in response to infection for Healthy and Asthmatic NECs. Mean ± SEM ,*p = <0.05. NT – no template control.