Figure 2

Differential cell distributions in EOC cases. (A) Estimated difference in leukocyte subtypes (i.e., CD8+ T-lymphocytes (CD8T), CD4+ T-lymphocytes (CD4T), natural killer cells (NK), B cells (Bcell), monocytes (Mono), and granulocytes (Gran)) between EOC cases and controls. Bars reflect the 95% confidence interval for the difference in cell distributions between EOC cases and controls. (B, C) Histograms of P-values obtained from examining the association between DNAm and EOC case/control status, (B) unadjusted for estimated cell distribution and (C) adjusted for the estimated cell distribution. Dashed line is the density histogram that is expected if all CpGs were null (not differentially methylated) and the dotted line is at the height of our estimate of the proportion of null p-values. (D, E) Volcano plots of –log₁₀(q-value) against the estimated difference in methylation between EOC cases and controls, (D) unadjusted for estimated cell distribution and (E) adjusted for the estimated cell distribution. Red and blue dashed lines indicate –log₁₀(q = 0.05) and –log₁₀(q = 0.10), respectively. Each model was fit to the combined data from the Batch 1 and 2 samples (n = 428) and were adjusted for age, smoking status, alcohol consumption, study enrollment year, location of residence, parity, and population substructure.