Table 2 Previously established definitions of four biological gold standards (GS) and the parameters of BLASTn results used in the theoretical predictions for identification of CSH probes (mouse probes used as an example).
Gold Standard | Parameters |
|---|---|
GS1.x | Top x ratio of relative expression (red/green) when exposed to erroneous RNA vs to correct RNA: |
GS2.x* | Top x% absolute expression when exposed to human RNA with mouse probes |
GS3.x | Top x% relative expression when exposed to human RNA with mouse probes (the expression of mouse probes when exposed to human RNA divided by the expression when exposed to mouse RNA) |
GS4.x | The same as GS2 but absolute log2 based expression on both green channels of human and mouse RNA expression of mouse probes > 4 |
Models | Conditions and parameters |
Model1* | {18<Align≤20 and mis<5} U {20<Align≤ 30 and mis<6} U {30<Align≤40 and mis<7} U {40<Align≤50 and mis<8} U {50<Align and mis<9} |
Model2 | {15 < Align ≤ 30 and mis < 2} U {30 < Align ≤ 40 and mis < 3} U {40 < Align ≤ 50 and mis < 5} U {50< Align and mis < 7} |
Model3 | Alig > 50 & mismatches < 6 |
Model4 | score > 70 & Alig > 58 & mis < 6 |