A systematic analysis of a broadly neutralizing antibody AR3C epitopes on Hepatitis C virus E2 envelope glycoprotein and their cross-reactivity

Table 2 Top ranked discontinuous peptides in the E2 protein dataset.

Rank	Discontinuous peptides	Frequency	Accumulative percentage	Validation status
1	ILNCNDSLGIALFYKCW	754	14.12%	Missing
2	ILNCNDSLGLALFYKCW	320	20.11%	Missing
3	ILNCNASLGIALFYKCW	256	24.91%	Missing
4	ILNCNDSLGLALFYRCW	240	29.40%	Neutralized
5	ILNCNASLGVALFYKCW	237	33.84%	Missing
6	ILNCNESLGLALFYKCW	221	37.98%	Neutralized
7	ILNCNDSLGIALIYKCW	213	41.97%	Missing
8	ILNCNASLGLALFYRCW	209	45.88%	Missing
9	ILNCNESIGIALFYKCW	173	49.12%	Missing
10	ILNCNDSLGIALFYRCW	152	51.97%	Missing
11	ILNCNDSLGLALFYNCW	141	54.61%	Neutralized
12	ILNCNDSLGLALFYSCW	127	56.99%	Neutralized
13	ILNCNDTIGIALFYRCW	127	59.36%	Missing
14	ILNCNDSIGIALFYRCW	123	61.67%	Missing
15	ILNCNDSLGIALLYKCW	102	63.58%	Missing
16	VLNCNESIGLALFYKCW	92	65.30%	Missing
17	ILNCNDSLGVALFYKCW	85	66.89%	Missing
18	ILNCNASLGLALFYKCW	85	68.48%	Missing
19	ILNCNASLGVALLYKCW	80	69.98%	Missing
20	ILNCNDSLGIALFYNCW	72	71.33%	Missing
21	ILNCDESIGIALFYKCW	71	72.66%	Missing
22	ILNCNDSIGIALFYKCW	71	73.99%	Missing
23	ILNCNESIGLALFYKCW	66	75.22%	Missing
24	ILNCNDSLGVALLYKCW	63	76.40%	Missing
25	LLNCNDSLGLALFYKCW	55	77.43%	Missing
26	ILNCNDSLGIALFYSCW	48	78.33%	Neutralized

The top 26 most frequent (including discontinuous peptides identical to discontinuous neutralized motifs) among 402 different patterns of discontinuous peptides are listed. This table lists discontinuous peptide, their frequencies, accumulative frequency and neutralization validation status to mAb AR3C. The neutralized discontinuous motifs are underlined. The residues in italics and underlined, indicate the amino acids that are not presented in the specific position from these known neutralized discontinuous motifs (see Figure 1(c)).

ISSN: 1755-8794