Figure 3

Gene by gene coverage of cardiac genes. A. Box-and-whisker plots showing median read depth and 25% to 75% interquartile ranges (IQR) for the 50 cardiac genes in 94 exome samples captured using Agilent V3 that had high observed sequencing depth, 80X. Plots represent the read depth coverage for transcripts found within the CCDS. There was variability in depth of coverage between genes and variable coverage within genes as shown by the wide IQR. Based on the CCDS dataset, 10 of the 50 genes had median read depth below 30X and only 32 genes had an IQR above 30X. B. Box-and-whisker plots showing median read depth and 25% to 75% interquartile ranges (IQR) for the 50 cardiac genes in 20 exome samples captured using Agilent V2 that had the lowest observed sequencing depth, 30.5X. Based on the CCDS dataset, 30 of the 50 genes had median read depth below 30X and only 5 genes had an IQR above 30X.