A representative differential spot among different NFPA subtypes versus control pituitaries (Spot-1311). Control means control pituitaries; LH means LH-positive NFPA; FSH means FSH-positive NFPA; LH/FSH means LH/FSH-positive NFPA; and NF means NFPA with negative hormone expression. A statistically significant difference existed between each type of NFPAs versus control pituitaries (p < 0.001). A. The 2DGE images (Spot-1311) among four NFPA subtypes. B. The normalized spot-volume (Spot-1311) among four NFPA subtypes.