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Figure 3 | BMC Medical Genomics

Figure 3

From: BMP2 response pattern in human lung fibroblasts predicts outcome in lung adenocarcinomas

Figure 3

Effects of BMP2 stimulation on CCL-171, MDA-MB321 and T47D cells. Biologically independent duplicate samples of CCL-171, MDA-MB-231 and T4D7 cells were exposed to BMP2 for 24 h. To determine the cell type specific gene expression changes, the mean of the mock gene expression profile was subtracted from mean of the gene expression profiles of the duplicates of the respective cell type induced by BMP2 for CCL-171, MDA-MB-231 and T47D. The profiles of induced or repressed genes were merged. Genes with a difference in expression of at least 4 fold were displayed after unsupervised hierarchical clustering in a heatmap. A set of genes specifically up-regulated in CCL-171, representing the “fibroblast specific BMP2 induced gene list” is indicated. In the zoomed image the genes of the “fibroblast specific BMP2 induced gene list” are shown for all duplicate samples. The array tree determined by unsupervised hierarchical clustering using average linkage of all the genes of the arrays is shown to demonstrate consistency of the duplicates. The mean expression values of all the genes of the “fibroblast specific BMP2 induced gene list” is shown above the cluster of all genes as expression level of the centroid.

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