Table 1 Numbers of bioinformatics analyses for each step are resumed
Genes differentially expressed | ||||
| Â | Day 0 | Day 7 | Day 14 | Total |
Probes | 250 | 317 | 246 | 536 |
Probes up-regulated (%) | 19 (7.6) | 52 (16.4) | 29 (11.8) | 79 (20.1) |
Probes down-regulated (%) | 231 (92.4) | 265 (83.6) | 217 (88.2) | 315 (79.9) |
Annotated genes | 159 | 215 | 184 | 350 |
Genes up-regulated (%) | 17 (10.7) | 46 (21.4) | 24 (13.0) | 67 (19.1) |
Genes down-regulated (%) | 142 (89.3) | 169 (78.6) | 160 (87.0) | 267 (80.9) |
miRs differentially expressed | ||||
| Â | Day 0 | Day 7 | Day 14 | Total |
miRs modulated | 7 | 4 | 16 | 22 |
miRs up-regulated (%) | 6 (85.6) | 1 (25) | 14 (87.5) | 16 + 3a |
miRs down-regulated (%) | 1 (14.3) | 3 (75) | 2 (12.5) | 3 + 3a |
Correlation and prediction methods | ||||
| Â | Day 0 | Day 7 | Day 14 | Total |
Total correlations | 1750 | 1268 | 3936 | 6954 |
Significant correlations (%) | 159 (9.1) | 136 (10.7) | 215 (5.5) | 510 (7.3) |
Negative correlations (%) | 119 (74.8) | 106 (77.9) | 70 (32.6) | 295 (57.8) |
Positive correlations (%) | 40 (25.2) | 30 (22.1) | 145 (67.4) | 215 (42.2) |
miRs differentially expressed with predicted targets in at least 1 database | 7 | 4 | 12 | 18 |
Total predictions at probe level | 96 | 181 | 461 | 738 |
Total predictions at gene level | 55 | 88 | 148 | 216 |
Merge of correlation and prediction methods | ||||
| Â | Day 0 | Day 7 | Day 14 | Total |
miRs | 2 | 2 | 6 | 9 |
Genes | 11 | 22 | 32 | 50 |
Significant couples miR-gene | 11 | 22 | 36 | 61 |