|
Method
|
Specificity
|
Throughput
|
Cost ($)
|
Study size
|
|---|
|
Novex TBE PAGE gel
|
High
|
Low
|
$$$$$
|
Small
|
|
(manually cutting band; very specific)
|
(few libraries/day)
| |
(2–10 samples)
|
|
Pippin Prep Automated gel system
|
Medium
|
Low
|
$$$
|
Medium
|
|
(automated band; less specific)
|
(4 libraries/run [2 hrs])
| |
(10–50 samples)
|
|
AMPure XP beads
|
Low
|
High
|
$
|
Large
|
|
(all products >100 nt)
|
(24 libraries/2 hrs)
| |
(50 and up)
|
- Recommendations for small RNA sequencing library purification. Recommendations include: (1) Specificity: based on specificity to a particular small RNA population. (2) Throughput: based on the number of libraries that can be prepared per day and efficiency of processing. This number is relative to the number of people working and instruments available in the lab. (3) Cost: based on price of reagents, hands-on laboratory time, service fees by genome centers. (4) Study Size: based on number of biological or technical replicates
