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Table 6 Sequencing coverage

From: Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing

Sample

D1-D12

D1-D12

Pearson (r)

RIN

7.4

7.4

-----

Sequencer

HiSeq2500

MiSeq

-----

Average Quality

37

36

-----

Raw Reads

11.556456

889645

-----

Size (<15 nt)

6.39 %

6.79 %

0.99039

Low Quality (Q <30)

1.68 %

1.33 %

0.95246

Adapter-Adapter

0.27 %

0.32 %

0.99639

RNAs >40 nt

37.63 %

33.13 %

0.98538

Surviving Reads

54.03 %

58.42 %

0.98834

Unmapped

6.01 %

5.61 %

0.99573

Uniq-Mapped

12.73 %

13.14 %

0.99653

Multi-Mapped

81.27 %

81.24 %

0.99672

miRNA

86.11 %

85.77 %

0.99374

Other ncRNAs

1.81 %

1.83 %

0.99512

Repeat

3.83 %

4.37 %

0.99679

Coding Gene

0.14 %

0.15 %

0.98360

Unknown

2.11 %

2.26 %

0.99109

miRNA Count (≥1)

563

231

0.99997

miRNA Count (≥10)

264

111

0.99997

miRNA Count (≥20)

217

92

0.99998

  1. Small RNA data analysis shows the percentage, composition and quality of reads from 12 libraries produced by our bioinformatics pipeline in order to test sequencing coverage for small RNA sequencing. Libraries were sequenced on both on HiSeq2500 and MiSeq Illumina sequencers
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BMC Medical Genomics

ISSN: 1755-8794

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