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Table 6 Sequencing coverage

From: Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing

Sample D1-D12 D1-D12 Pearson (r)
RIN 7.4 7.4 -----
Sequencer HiSeq2500 MiSeq -----
Average Quality 37 36 -----
Raw Reads 11.556456 889645 -----
Size (<15 nt) 6.39 % 6.79 % 0.99039
Low Quality (Q <30) 1.68 % 1.33 % 0.95246
Adapter-Adapter 0.27 % 0.32 % 0.99639
RNAs >40 nt 37.63 % 33.13 % 0.98538
Surviving Reads 54.03 % 58.42 % 0.98834
Unmapped 6.01 % 5.61 % 0.99573
Uniq-Mapped 12.73 % 13.14 % 0.99653
Multi-Mapped 81.27 % 81.24 % 0.99672
miRNA 86.11 % 85.77 % 0.99374
Other ncRNAs 1.81 % 1.83 % 0.99512
Repeat 3.83 % 4.37 % 0.99679
Coding Gene 0.14 % 0.15 % 0.98360
Unknown 2.11 % 2.26 % 0.99109
miRNA Count (≥1) 563 231 0.99997
miRNA Count (≥10) 264 111 0.99997
miRNA Count (≥20) 217 92 0.99998
  1. Small RNA data analysis shows the percentage, composition and quality of reads from 12 libraries produced by our bioinformatics pipeline in order to test sequencing coverage for small RNA sequencing. Libraries were sequenced on both on HiSeq2500 and MiSeq Illumina sequencers