Skip to main content
Fig. 2 | BMC Medical Genomics

Fig. 2

From: FoxM1 transactivates PTTG1 and promotes colorectal cancer cell migration and invasion

Fig. 2

FoxM1 induces PTTG1 mRNA and protein expression in colon cancer cells. FoxM1 siRNA suppressed PTTG1 mRNA expression. SW620 (a) and HCT116 (b) cells were transfected with negative control siRNA (Con), FoxM1 siRNA (siFoxM1), negative control pcDNA3.1 (Con) or FoxM1 plasmid (FoxM1), respectively. FoxM1 and PTTG1 mRNA levels were detected by real-time PCR; results were normalized with controls and expressed as mean ± SD from triplicate assays. FoxM1 siRNA inhibited, and over-expression increased PTTG1 protein expression. SW620 (c) and HCT116 (d) cells were transfected with negative control siRNA (Con), FoxM1 siRNA (siFoxM1), negative control pcDNA3.1 (Con) or FoxM1 plasmid (FoxM1), respectively. FoxM1 and PTTG1 protein levels were measured by western blot. All western blot experiments were repeated three times. Results of a representative experiment are shown. *p < 0.05, **p < 0.01, ***p < 0.001

Back to article page