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Table 1 Primers used for real-time PCR and real-time PCR validation of RNA-seq results

From: RNA sequencing from human neutrophils reveals distinct transcriptional differences associated with chronic inflammatory states

Gene ID

Primer direction

Primers sequence (5′ ~ 3′)

Fold change (AD vs CRM)

RNA-seq

qPCR

DDX60

Forward

GAA GCA GCA GGA AGC TGA A

−5.73

−1.26

 

Reverse

GGA TGT CTC TCA GTT GCT CAA A

  

IFIH1

Forward

TTG GAT AAG TGC ATG GAG GAG

−3.61

−1.53

 

Reverse

CCT GTT TGA CGA AGA ACA TTC AG

  

IFITM3

Forward

CCT GTT CAA CAC CCT CTT CA

−5.30

−1.82

 

Reverse

CAT GAG GAT GCC CAG AAT CA

  

IGHMBP2

Forward

ACG AAC AGT CGA AAG GGA AC

2.99

1.10

 

Reverse

AGC CAT CGA CAG ACT TGA TTT

  

MOV10

Forward

GGG CTA TGA CCT GGA GTT AAG

1.44

−3.50

 

Reverse

CAC CTC ATA GTT CCT CCA CTT C

  

OAS1

Forward

GAA GCC TGT CAA AGA GAG AGA G

−13.46

−1.24

 

Reverse

GTT AGG TTT ATA GCC GCC AGT

  

PML

Forward

ACA ACA TCT TCT GCT CCA ACC

−2.64

2.21

 

Reverse

TGT CGC TGC TGG ATC TCT

  

RNF213

Forward

CTG GTT GTG TCA CCT CCT AAC

−3.07

1.46

 

Reverse

GTC CTT GTG TCC ATG CAT CT

  

TNFAIP6

Forward

GAT GGG ATG CCT ATT GCT ACA

−2.79

−3.70

 

Reverse

CGC TGA CCA TAC TTG AGT CTA AT

  

TRIM5

Forward

GCA GGA AGC TGA AGA GTT AGA

−1.33

−2.58

 

Reverse

GAA TGT CTT CCT CCT CCT TCT C

  

lnc-CKAP2L-1

Forward

GTTAAAGCTGCGAAGAACCTAAC

13.94

1.19

 

Reverse

TTCCTGCCTCTTCCTACTCT

  

lnc-IFITM2-4

Forward

GATCTTAGCCTTGGCCTCAC

−7.98

−24.85

 

Reverse

TACACCAGGCAACCACAAATA

  

lnc-IRS2-2

Forward

GCTAGTTCAGCCTGTGAGATG

17.2

6.69

 

Reverse

AGCAAGCAATCCAAGAGAGAG

  

lnc-PFDN4-1

Forward

GGTGTTTGGAGACAAAGGAATAG

3.77

1.28

 

Reverse

CTATCTCGTGCCGCTTAGTATC

  

lnc-PML-1

Forward

TGTAGCACTCACGGCAAAT

−6.16

−2.39

 

Reverse

CGTGTCCAGAGTTTGTTCCT

  

lnc-RBL2-1

Forward

TCCTGAGTAGCTGGGAT GTA

−2.2

−1.61

 

Reverse

GACCAGCCTAGCCAACATAAT

  

lnc-SLC2A13-1

Forward

TAATGGCAGTGGAGGTTGTC

−2.78

−3.39

 

Reverse

GAACTTCCAGCATCTCCTTACA

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