Fig. 5

Tissue/cell markers in AF collected at different stages of pregnancy were identified. a Expression distribution analysis was used to identify genes expressed greater than 90 % quantile in Prenatal, Late Preterm or Term AF samples. Tissue specific gene markers were identified to meet two criteria: 1) the gene expression in tissue A should be at least 3 fold higher than its average expression across 66 tissues, and 2) the gene expression in tissue A should be at least 1.5 fold higher than any of the 66 tissues. Relative amount of tissue selective gene markers expressed in AF at the three gestational age periods were calculated and shown in bar graph. The portion of tissue specific genes in Prenatal samples is noted in Green, Late Preterm in Red, and Term in Blue. b Using the software GO-Elite, tissues and cell-types predicted to be enriched (Fisher’s Exact p <0.05) were identified and visualized as a network along with associated marker genes in AltAnalyze