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Fig. 5 | BMC Medical Genomics

Fig. 5

From: Histone methylation levels correlate with TGFBIp and extracellular matrix gene expression in normal and granular corneal dystrophy type 2 corneal fibroblasts

Fig. 5

TGFβ1 increased H3K4me1/3 levels on TGFBIp and ECM-associated gene promoters in wild-type and GCD2-homozygous cells. a, b Bar graphs showing H3K4me3 (a) and H3K4me1 (b) levels at the indicated gene promoters in control and TGFβ1 (5 ng/ml)-stimulated wild-type and GCD2-homozygous corneal fibroblasts. ChIP assays were performed with H3K4me3 and H3K4me1 antibodies. Immunoprecipitated DNA and input DNA were subjected to qPCR with primers specific for the indicated gene promoters to measure enrichment levels. qPCR data were analyzed using the 2-ΔΔCt method, and data are shown as the mean fold change of the input ± standard error (SE) of enrichment. (mean ± SE; **P < 0.01 vs. wild type, n = 3)

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BMC Medical Genomics

ISSN: 1755-8794

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