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Fig. 6 | BMC Medical Genomics

Fig. 6

From: Histone methylation levels correlate with TGFBIp and extracellular matrix gene expression in normal and granular corneal dystrophy type 2 corneal fibroblasts

Fig. 6

TGFβ1 enhanced MLL1 recruitment on TGFBIp and ECM-associated gene promoters. a Total mRNA was isolated from wild-type and GCD2-homozygous corneal fibroblasts, and gene-expression profiles were assessed using an Affymetrix gene chip. The expression levels of H3K4 methyltransferase genes are presented as the log2 ratio. b, c MLL1 recruitment to the indicated gene promoters in wild-type and GCD2 corneal fibroblasts stimulated by TGFβ1 (5 ng/ml) for 8 h. MLL1 recruitment was determined by ChIP assays with MLL1 antibody. Results were normalized to the input, and MLL1 occupancy was expressed as the fold enrichment over the respective wild-type control samples (mean ± standard error (SE); **P < 0.01 vs. wild type, n = 3)

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BMC Medical Genomics

ISSN: 1755-8794

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