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Fig. 3 | BMC Medical Genomics

Fig. 3

From: IL-10Rα expression is post-transcriptionally regulated by miR-15a, miR-185, and miR-211 in melanoma

Fig. 3

IL-10Rα is the direct target of miR-15a, miR-185, and miR-211 a Schematic representation of the predicted interaction of miR-15a, miR-185, and miR-211 with IL-10Rα 3′UTR site. b Luciferase reporter assay was performed to detect the effect of individual or combined miRNA mimics on the luciferase intensity controlled by 3′UTR of IL-10Rα. Data are depicted as the mean ± SD of three independent experiments. Significant ***p < 0.001 and ****p < 0.0001, as compared to cells transfected with mimic control. c Luciferase reporter assay was performed to detect the effect of individual or combined miRNA inhibitors on the luciferase intensity controlled by 3′UTR of IL-10Rα. Data are depicted as the mean ± SD of three independent experiments. Significant ***p < 0.001 and ****p < 0.0001, as compared to cells transfected with inhibitor control. d Cells were transfected with individual or combined miRNA mimics and subjected to western blot for IL-10, IL-10Rα, and IL-10Rβ protein expression. β-actin was used as an internal control for normalization. Blots are representative of three independent experiments. The relative densities were calculated by dividing the density of IL-10Rα bands by the density of β-actin band at the same point. Significant **p < 0.01 and *** p < 0.001, as compared to cells transfected with mimic control

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