Fig. 5

Effects of miR-15a, miR-185, and miR-211 on IL-10-induced melanoma cell growth. a Cells were cultured in the presence of various doses of rIL-10: 0 (as negative control), 50, 100, or 500 U/ml and proliferation was assessed by MTT assay. Absorbance at 570 nm was measured at 6-hr intervals over a 72-hr culture period. Each experiment was repeated three times, and data represent the mean ± SD of three determinations. Significant **p < 0.01 and ***p < 0.001, cells treated with 100 U/ml as compared to the negative control at the same time point. Significant °p < 0.05 and °°p < 0.01, cells treated with 500 U/ml as compared to the negative control at the same time point. b Cells were transfected with individual or combined miR-15a, miR-185, and miR-211 mimics for 48-hr and then treated with rIL-10 (100 U/ml) for 36-hr. Proliferation was determined as reported in section A. Significant **p < 0.01 and ***p < 0.001, as compared to untransfected cells. c Cells were transfected with individual or combined miR-15a, miR-185, and miR-211 inhibitors for 48-hr and, where indicated, co-transfected with siRNA against IL-10Rα or non-targeting control siRNA. Then, cells were treated with rIL-10 (100 U/ml) for 36-hr. Proliferation was determined as reported in section A. Significant **p < 0.01 and ***p < 0.001, as compared to untransfected cells