Table 2 Primers designed for qRT-PCR validation of candidate lncRNAs and β-actin
lncRNA Accession Number | Forward primer | Reverse primer | Product length (bp) | Tm (°C) |
|---|---|---|---|---|
n345630 | 5'-TCCGTTGAACCTTCCACAGT-3' | 5'-ACTCTGCTCCGTTCCACATT-3' | 168 | 58 |
TCONS_00010304-XLOC_004787 | 5'-CTCAGGAAAGGAGTATAGAATG-3' | 5'-GGTGCAAGGTATAGAGTGT-3' | 104 | 58 |
n378726 | 5'-CCACAATGCAAACAACTGCT-3' | 5'-GAAAGCTGCTCTGTGGTGAA -3' | 161 | 60 |
NR_026860 | 5'-CTTGGTTGTGCGGGATTCT-3' | 5'-GTCAGAAGGAGGAGCAGGTAG-3' | 204 | 60 |
n345729 | 5'-AGGGTCATTTAGCCAGAAAGT-3 | 5'-GATAAACCCAGATGCCCTTGTAG-3' | 144 | 58 |
n342056 | 5'-CAGGCTTATGGAGCGTTAAGAAT-3' | 5'-CATCAGGGAGAAGTTATCAGGT-3' | 177 | 58 |
n384667 | 5'-TGCCTGATAAGGTCACATACAC-3' | 5'-CCAGGACATGCGATGAAGATTG-3' | 123 | 58 |
TCONS_00011401-XLOC_005517 | 5'-TCCTGGGTCAAGCTGAGTATC-3' | 5'-TGGAGTCTTACAAATCTTTTA-3' | 131 | 58 |
NR_038366 | 5'-ACTCCGTGTTACTCATTCC-3' | 5'-TTGCTTCTTCTTCTCCTCTT-3' | 188 | 58 |
NR_024204 | 5'-GAATAACTGGGAGATGAAACAGG-3' | 5'-CAACAGGTAGAGGTGCTGGA-3' | 102 | 58 |
NR_015379 | 5'-TCCATTCAGACCGCCACTCAC-3' | 5'-CAAGGTGCCAGTTAGCGTAT-3' | 244 | 58 |
TCONS_00027385-XLOC_013370 | 5'-GGCTGTCTTAGAAGGATGAA-3' | 5'-AATAGAGCTGGTTGACTGC-3' | 129 | 58 |
n408024 | 5'-CGGAAGGTTACAGTCTCTAG-3' | 5'-TGCTGTGTCCTCATTTATCA-3' | 125 | 58 |
β-actin | 5'-GATGACCCAGATCATGTTTGAG-3' | 5'-AGGGCATACCCCTCGTAGAT-3' | 159 | 58-60 |