Fig. 2
Differential expression of innate immune inflammatory response genes in human necrotizing enterocolitis. Real-time qPCR analysis of transcript levels of selected target genes related to intestinal innate immunity. Ct values of selected genes were normalized using B2M as reference gene and data are expressed as r∆Ct values (reverse ∆Ct: Ctreference gene-Ctgene of interest) in order to display direct variation in NEC vs non-NEC controls (CTRL). Horizontal line represents the median value of r∆Ct values for CTRL and NEC samples. *: p <0.05 between CTRL and NEC samples. Numbers indicated represent the fold variation between NEC and CTRL