Fig. 3

Protein expression of MPP7 in normal HTM cells and down-regulation of mRNA under cyclic mechanical stress. a The immunoblot shows the expression of MPP7 in HTM cell line. Thirty microgram protein was loaded on gel. β-Actin was used as loading control. Three independent experiments were done to confirm the expression. b This plot represents the expression of MPP7 gene transcripts in primary HTM cells with (HTM_stressed) and without cyclic mechanical stress (HTM_control) in a publicly available dataset (GSE14768). X-axis represents MPP7 shows down-regulation under cyclic mechanical stress with significant FDR-corrected p-value of 0.018 and fold change in stressed HTM was observed to be 2.6 (log Fc = −1.38)