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Table 2 CpG sites significantly correlated with SBP and EH

From: Longitudinal genome-wide methylation study of Roux-en-Y gastric bypass patients reveals novel CpG sites associated with essential hypertension

    Discovery groupa (n=11) Verification cohortb (n=539)
    % DNA Methylation (SD) Spearman’s rank correlation coefficients (SBP)   Binary logistic regressions (EH)
Gene Illumina ID Distance to TSS BaselineA After surgeryB p A vs. B Coef. p p (Bonf.) % DNA Methylation (SD) Exp (B) p p (Bonf.)
MCCC1 cg00161968 27 5.36 (1.1) 5.9 (1.7) ns 0.93 <10E–16 <10E–16 7.5 (4.3) ns ns
SKOR2 cg00875989 1500 33.2 (1.7) 33.6 (3.6) ns (0.92) <10E–16 <10E–16 37.5 (4.2) 0.92 1.16E–03 2.79E–02
VGLL3 cg06251539 375 9.3 (2.4) 8.7 (1.88) ns 0.92 <10E–16 <10E–16 14.5 (3.1) ns ns
WSB1 cg08706258 125 4.3 (1.1) 4.2 (0.7) ns 0.93 <10E–16 <10E–16 4.8 (0.8) ns ns
EHMT2 cg09134341 (1332) 91.0 (2.8) 91.8 (1.2) ns (0.92) <10E–16 <10E–16 89.3 (1.9) 0.84 9.58E–04 2.30E–02
TCF12 cg10146710 1132 2.4 (0.5) 2.4 (0.4) ns 0.92 <10E–16 <10E–16 2.3 (1.0) ns ns
MAP2K4 cg10596925 (242) 12.1 (3.4) 13.0 (2.0) ns 0.95 <10E–16 <10E–16 10.7 (2.9) 0.94 4.98E–02 ns
FAM54A cg10640093 (196) 3.8 (1.0) 4.1 (0.7) ns 0.94 <10E–16 <10E–16 3.0 (0.7) ns ns
SORBS1 cg12360759 598 7.4 (1.7) 7.4 (1.0) ns 0.92 <10E–16 <10E–16 9.1 (1.3 0.82 6.33E–03 ns
DDX55 cg15612682 232 6.0 (1.5) 6.1 (0.8) ns 0.93 <10E–16 <10E–16 5.6 (1.0) ns ns
ATXN1L cg16076930 380 19.0 (2.8) 20.5 (5.1) ns 0.94 <10E–16 <10E–16 21.1 (2.8) ns ns
EFEMP1 cg16118212 227 12.7 (4.4) 10.7 (2.9) ns 0.92 <10E–16 <10E–16 17.3 (6.2) ns ns
CRKL cg16500810 (197) 1.5 (0.3) 1.6 (0.9) ns 0.92 <10E–16 <10E–16 0.6 (0.6) ns ns
STEAP3 cg18643762 (211) 2.9 (1.0) 3.4 (1.0) ns (0.93) <10E–16 <10E–16 1.7 (1.3) ns ns
CPEB4 cg20841073 648 10.7 (2.2) 10.3 (2.7) ns 0.93 <10E–16 <10E–16 5.2 (2.0) 0.85 2.37E–03 ns
RHOBTB2 cg21344124 302 3.2 (0.7) 2.8 (1.1) ns 0.92 <10E–16 <10E–16 2.2 (0.7) 1.34 1.96E–02 ns
ATXN1 cg21996137 (185) 2.0 (0.7) 1.8 (0.6) ns 0.92 <10E–16 <10E–16 1.6 (0.7) 1.42 1.60E–02 ns
TSSK1B cg22011370 525 89.9 (2.6) 89.9 (2.9) ns (0.95) <10E–16 <10E–16 90.6 (3.3) ns ns
SPPL2A cg22295383 (104) 5.2 (1.3) 4.9 (1.1) ns 0.94 <10E–16 <10E–16 4.2 (1.3) ns ns
BHLHA9 cg23945265 1003 45.4 (4.2) 46.9 (3.2) ns (0.92) <10E–16 <10E–16 45.7 (5.2) 0.96 2.58E–02 ns
CHCHD5 cg25521086 (58) 4.0 (0.8) 3.8 (0.7) ns 0.95 <10E–16 <10E–16 3.1 (1.2) ns ns
NRTN cg25544164 342 99.0 (0.3) 9.1 (0.2) ns 0.94 <10E–16 <10E–16 99.1 (0.5) ns ns
  1. Abbreviations: EH essential hypertension, Exp(B) log odds ratio, ns not significant, RYGB Roux-en-Y gastric bypass, SBP systolic blood pressure, SD standard deviation, TSS transcription start site
  2. aThis group comprises patients who underwent RYGB surgery. bResults were derived from binary logistic regressions of EH (yes/no) to % CpG site methylation, age, BMI, ethnicity and sex. Log odds ratios and p-values refer to the CpG site in focus. P-values for DNA methylation levels were calculated by paired t-tests, contrasting methylation levels at baseline and six months after RYGB-surgery (p: A vs. B). GWAS results for SBP were calculated by genome-wide analyses of Spearman's rank correlation coefficients using algorithm AS 89 and an Edgeworth series approximation. Unless specified as SD, parenthesis “()” implies negative numbers