Fig. 3

Partial Least Squares Discriminant (PLSD) Model for classification of appendicitis from RNA biomarkers. DEGs were analyzed by PLSD to compose a classification model for appendicitis based on RNA biomarkers in blood. The 3D plot shows the 20 patients in the discovery set as partitioned by the first 3 of 4 latent factors in the PLSD model. The red spheres represent abdominal pain patients (n = 9), and blue shows the cluster of appendicitis patients (n = 11), as a function of the t0 latent factor (X-axis), the t1 factor (Y-axis), and the t2 factor (Z-axis). The majority of patients (7/9) are accurately classified by the t0 component alone