Fig. 5
From: Computational analysis of the mesenchymal signature landscape in gliomas
CD44 inhibition is associated with decreased cell viability and migration/invasion in GBM cancer and stem cells. a Western blot analysis showing differential expression of CD44 in GBM tumor (LN18, U87, and U251) and stem cells (923, GBAM1, GBMJ1, and NSC11). Analysis of siCD44 transfected U251 cell line showed b substantial inhibition of CD44 expression 24 h post transfection using 2 different siRNAs, c significant time course reduction of cell viability, and (d) inhibition of long term survival using clonogenic assay. e A representative wound healing assay showing diminished migration of U251 cells in siCD44 transfected cells compared to control. f-h. Boyden chamber assay showing F. decreased ability of U251 cells transfected with CD44siRNA through the matrigel coated membrane compared to control. Chemoattractant was 1% FBS. Quantified data depicted in (g). and bars represent the mean of 3 independent experiments measured in triplicate +/- standard deviation (h). Stem cells shown to have minimal CD44 expression had a decreased ability to migrate towards U251 cells compared to negative transfected control. Chemoattractants were FBS and stem cell growth factors (bFGF and EGF)