Fig. 1

Volcano plots are shown highlighting gene expression differences between dystrophic medial head of the gastrocnemius (MHG) skeletal muscle of golden retriever muscular dystrophy (GRMD) dogs at 6 (a) and 12 (b) months (m), as well as GRMD left ventricle (LV) from 6 (c) and 12 (d) month-old dogs, each compared to their respective wild type controls. The y-axis displays p values, and fold-differences are plotted on the x-axis for GRMD versus control dogs for each of the four comparisons. Each circle represents an individual probe set (gene) and is colored based on fold-difference (blue for genes with lower expression in GRMD dogs, red for genes with higher expression values, and gray for no change), as shown in the legend. Orange lines mark significance cut-off values (p value < 0.05, fold-difference > 1.5). Panel (e) shows a matrix including the number (No.) of array signal intensity probes deemed as significantly differential (p value < 0.05, fold-difference > 1.5) between pairs of groups. Pairwise comparisons include GRMD versus wild-type (WT) dogs for matched skeletal muscle (MHG) and LV tissue of dogs aged 6 months and 12 months. Pairwise comparisons of the two age groups for each tissue are also shown