Fig. 4

MiR-30a positively regulates the expression of PTEN in MG-63 cells. a MG63 cells were transfected with miR-30a-3p mimic and mimic control for 24 h. The expression levels of PTEN were determined by western blotting analysis. The gray values of PTEN were shown in histogram. **p < 0.01, compared with mimic negative control group. b MG63 cells were transfected with miR-30a-3p inhibitor and inhibitor negative control for 24 h. The level of PTEN was determined by western blotting analysis. There was statistical difference between miR-30a-3p inhibitor transfected cells and inhibitor negative control transfected cells (**p < 0.01). c MG-63 cells were pretreated with 500 nM SF1670 for 3 h and then, transfected with miR-30a-3p mimic and mimic control for 24 h. The expression levels of PTEN were determined by western blotting analysis. The gray values of PTEN were shown in histogram. **p < 0.01, compared with mimic negative control group