Fig. 3
From: Targeted sequencing reveals complex, phenotype-correlated genotypes in cystic fibrosis

Impact of the primer trimming on variant calling. Detection of the p.Glu92Lys variant was complicated by presence of the technical sequences in data, which lead to false negative calls employing standard pipelines: a. Technical sequences (primers) result in reference allele overestimation and influence variant calling b. Trimming primers allow to detect mutation in all samples