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Table 2 Under and overexpressed proteins identified by two-dimensional gel electrophoresis (2-DE) in metastatic (N+) and non-metastatic (N0) lymph nodes from oral cavity SCC patients. Proteins were separated by two-dimensional electrophoresis and identified by MALDI-Q-TOF MS/MS. Twenty-two proteins were considered with differential abundance between the categories (Student’s t test p < 0.05). C02, C04, C32 columns correspond to pools from tongue (pools A and B), floor of the mouth (pools C and D) and larynx carcinomas (pools E and F), respectively. N+/N0: abundance ratio

From: Differentially expressed proteins in positive versus negative HNSCC lymph nodes

Protein name Gene symbol UniProt accession pI Mass Sequence coverage (%) Score Queries matched Areaa C02 C04 C32 N+
N+/N0
Carbonic anhydrase 1 or CA-I CA1 P00915 6.65 28,620 16 116 3 VII 0.4402 0.2033 0.2971 Down
Calreticulin or CRP55 CALR P27797 4.29 48,283 9 167 3 IX    0.6211 Down
Hemoglobin subunit delta HBD P02042 7.97 16,028 19 64 2 IV   0.3906 0.1063 Down
Heat shock protein 90 kDa beta member 1 or endoplasmin HSP90B1 P14625 4.76 92,696 4 69 3 I 0.4010   0.5364 Down
Cytosol aminopeptidase or LAP-3 LAP3 P28838 6.29 53,006 4 71 3 IX 0.4514   0.6685 Down
Protein disulfide-isomerase or PDI P4HB P07237 4.76 57,480 8 201 4 IX    0.6616 Down
Profilin-1 PFN1 P07737 8.48 15,085 10 50 2 IV 0.3568 0.4595 0.6419 Down
Triosephosphate isomerase or TIM TPI1 P60174 6.51 26,807 8 51 1 VI 0.5020 0.3262 0.3373 Down
Aldo-keto reductase family 1 member B10 or ARL-1 AKR1B10 O60218 7.12 36,226 12 149 3 II 3.7339 2.2564   Up
Apolipoprotein A-I or Apo-AI APOA1 P02647 5.56 30,759 11 104 3 VI   1.5333   Up
Cystatin-B or CPI-B CSTB P04080 7.90 11,224 24 64 2 XI 2.0971 1.9614   Up
Fatty acid-binding protein, epidermal or E-FABP FABP5 Q01469 6.84 15,366 18 94 2 VIII 4.0016 7.4081 5.5954 Up
Glutathione S-transferase P or GSTP1–1 GSTP1 P09211 5.44 23,438 12 78 2 VI 2.3289 4.0385 2.9731 Up
Heat shock protein beta-1 or HspB1 HSPB1 P04792 5.98 22,826 20 105 3 III 3.2028 4.1256 4.5202 Up
Ig gamma-1 chain C region IGHG1 P01857 8.46 36,596 9 71 2 X 4.1259   4.3046 Up
Ig kappa chain C region IGKC P01834 7.55 26,077 14 48 2 VII   2.5279 2.1303 Up
Keratin, type II cytoskeletal 1 or CK1 KRT1 P04264 8.16 66,018 4 82 2 VII   1.7525 2.0958 Up
Galectin-1 or Gal-1 LGALS1 P09382 5.34 14,917 8 54 1 V 1.8654 2.2229 1.4698 Up
Galectin-7 or Gal-7 LGALS7B P47929 7.00 14,992 19 73 2 VIII 6.2867 2.5605 2.7244 Up
Protein S100-A7 or psoriasin S100A7 P31151 6.26 11,433 34 103 3 VIII 6.4075 9.3486 5.3993 Up
Protein S100-A9 or calgranulin-B S100A9 P06702 5.71 13,291 42 100 3 VIII 4.7500 5.9737 4.9505 Up
14–3-3 protein sigma or stratifin SFN P31947 4.64 27,874 15 98 3 III 13.4829 7.3989 27.6468 Up
  1. aAreas are numbered as in Additional file 1