Table 1 Primer information: sequences and length of the amplified fragment
| Gene | RefSeq_ID | Primer sequences (sense and antisense) | Size |
|---|---|---|---|
| COL10A1 | NM_000493.3 |
5′- CCTACTCCTTATTTACGACGCAAT-3′ 5′- TGAAAAGCCTTGAAAGAATGG-3’ | 107 bp |
| COL11A1 | NM_001854.2 |
5’-TGATAATTTATGACAAAAGAACATACC-3′ 5’-CCAGGTAGCCAAGACTTGAGTTTA-3’ | 94 bp |
| CSPG2 | NM_004385.2 |
5’-GAATGGGATCCTGATGGAAC-3′ 5’-AGTCCTCCATTCAGGCCTTT-3’ | 96 bp |
| GREM1 | NM_013372.5 |
5’- TCATTTAAAAACGGCAAAGAA-3′ 5′- TTCATGAAACTTGAAGCCAAA-3’ | 111 bp |
| KRT14 | NM_000526.3 |
5’- CAGATCCCACTGGAAGATCC-3′ 5′- AAGCTGTATTGATTGCCAGGA-3’ | 92 bp |
| MMP11 | NM_005940.3 |
5’- AATCCAGGCCAAAAAGTTCA-3′ 5′- CCTGGGACAGGATTGAGGTA-3’ | 100 bp |
| PLEKHC1 | NM_006832.1 |
5’-GGCCATGTTCTAGTCTGTTGC-3′ 5’-CTCTCCCTCGCACCCTTT-3’ | 93 bp |
| SULF1 | NM_015170.1 |
5’- CAAATTAGCTGCTTGCCTGA-3′ 5′- AACTTGAAATCTTTTTACAAAGCACA-3’ | 99 bp |
| THBS2 | NM_003247.2 |
5’-AGGTTGATGAAACGTCATGTG-3′ 5’-AAGTGCAGGGTTTCAGTGGT-3’ | 93 bp |
| GAPDH | NM_002046.3 |
5’-CTCCTCACAGTTGCCATGTA-3′ 5′- GCACAGGGTACTTTATTTGATGG-3’ | 90 bp |
| ACTB | NM_007393 |
5’-TCCCCCAACTTGAGATGTATGAAG-3′ 5’-AACTGGTCTCAAGTCAGTGTACAGG-3’ | 90 bp |
| YWHAZ | NM_003406.2 |
5’- TGGAGGGTCGTCTCAAGTAT-3′ 5′- GCTCCGTCTCAATTTTCTCTC-3’ | 94 bp |