Fig. 1

Schematic representation of experimental approach. The calling card system was used to identify regions of genomic interactions by BAP1. Plasmids were constructed to express BAP1 fused with the piggyBac transposase (PBase) via an 18-aa linker. After plasmid transfection into UM cells, expressed BAP1-PBase fusion proteins interact with nucleosomes containing ubiquitylated histone H2A via BAP1, allowing PBase to insert a barcoded transposon from a co-transfected donor plasmid into the adjacent genomic DNA. The genome is fragmented, and barcoded transposon primers are used to amplify the genomic insertion sites for each transposon. Next-gen sequencing is performed to identify the genomic insertion sites, which are mapped back to the hg38 human genome assemble