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Fig. 2 | BMC Medical Genomics

Fig. 2

From: Genomics of lipid-laden human hepatocyte cultures enables drug target screening for the treatment of non-alcoholic fatty liver disease

Fig. 2

Effects of dynamin and FATP2 inhibitors on hepatic lipid uptake and Venn diagram of commonly regulated genes in steatotic primary human hepatocyte and human hepatoma cell cultures. A1 HepG2 cell cultures were treated with either DMSO or FA or FA and dynamin (=LDLR blocker) for 1 h. The fluorescence images were captured at 10x with a Nikon TiE phase contrast microscope and analyzed with the NIS elements software version 4.13. A2 When compared to the FA treatment alone the combined treatment of HepG2 cell cultures with FA and the dynamin inhibitor dynasore reduced significantly hepatic fatty acid uptake (p < 0.001). B1 HepG2 cell cultures were treated with either DMSO or FA or FA and the FATP2 blocker CB16.1 for 1 h. The fluorescence images were captured at 10x with a Nikon TiE phase contrast microscope and analyzed with the NIS elements software version 4.13. (B2) When compared to the DMSO vehicle (K+) control the combined fatty acid (FA) and FATP2 inhibitor treatment reduced significantly hepatic fatty acid uptake (p < 0.001). The symbol K- refers to cell cultures without treatment of the DMSO vehicle control. The ORO lipid stain was observed with the Texas Red filter; the size bar refers to 100 μm. *** denotes a significant p-value < 0.001. c Depicted is a comparison of PHH, HepG2 and HuH7 cells treated with a 1:1 mixture of 0.5 mM PA/OA for 72 h. HSDL2 was commonly regulated. d Depicted is a comparison of PHH, HepG2 and HuH7 cells treated with a 1:1 mixture of 0.5 mM PA/OA and TNFα for 72 h. HSDL2 and PDK4 were commonly regulated

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