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Fig. 5 | BMC Medical Genomics

Fig. 5

From: Genomics of lipid-laden human hepatocyte cultures enables drug target screening for the treatment of non-alcoholic fatty liver disease

Fig. 5

Immunofluorescence imaging of HILPDA and ROCK2 in Huh7 cells. Cultures of the human hepatoma cell line Huh7 were treated with a mixture of 0.5 mM PA/OA or the combined lipid and TNFα treatment and stained individually for HILPDA or ROCK2. Lipids were stained with the blue fluorescent dye MDP. a HILPDA (red) and lipid droplets (blue). Shown in the upper panel is the lipid stain; in the second panel HILPDA expression is given and the lower panel depicts the merged images. b ROCK2 (red) and lipid droplets (blue). Shown in the upper panel is the lipid stain; in the second panel ROCK2 expression is given and the lower panel depicts the merged images. The images were captured with Nikon TiE inverted microscope (images were captured with a 60x objective and the scale bar is given in μm)

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