Fig. 6

Immunofluorescence imaging of Hif1, PDK4 and JAK1 in primary human hepatocyte cultures. Cultures of primary human hepatocytes were treated with a mixture of 0.5 mM PA/OA or the combined lipid and TNFα treatment and stained individually for HIF1α, PDK4 or JAK1. Lipids were stained with the blue fluorescent dye MDP. Depicted are merged images. a HIF1α (red) and lipid droplet (blue). b PDK4 (green) and lipid droplets (blue). c JAK1 (red) and lipid droplets (blue). The images were captured with Nikon TiE inverted microscope (images were captured with a 60x objective and the scale bar is given in μm)