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Fig. 9 | BMC Medical Genomics

Fig. 9

From: Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells

Fig. 9

Positions of chromosome breaks within the non-SAR region in BA-treated TWO4 cells. The genomic map of AF9 non-SAR region from nucleotide positions 71,116–75,277 is illustrated above [EMBL: ENSG00000171843]. BamH I (B), Hind III (H) and Xba I (X) restriction sites are shown. Green arrows represent the primers (R1, AF9 71,653 R and F1, AF9 74,399 F) used in the first round of nested IPCR while blue arrows represent the primers (R2, AF9 71,282 R and F2, AF9 74,494 F) used in the second round of nested IPCR. Black boxes represent the repeat elements. Red and green vertical lines represent the presently detected breakpoints in TWO4 cells upon BA treatment at neutral and acidic pH, respectively. Five chromosome breaks (at coordinates 74,908, 74,914, 74,953, 74,985 and 74,987) fall within the first repeat CHARLIE5 (at coordinates 74,895–74,998). One chromosome break (at coordinate 75,043) falls within the second repeat CHARLIE5 (at coordinates 75,006–75,169)

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BMC Medical Genomics

ISSN: 1755-8794

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