Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells

Table 6 Topo II consensus sites predicted in the AF9 SAR and non-SAR regions

Topo II sites	DNA sequence 5’RNY NNCNNGYNGKTNYNY 3′	Percentage of matching nucleotides (%)		Nucleotide position of topo II sites
Topo II sites	DNA sequence 5’RNY NNCNNGYNGKTNYNY 3′	W Strand	C Strand	Nucleotide position of topo II sites
AF9 SAR region (nucleotide position 236,059–246,292)
1	W: AACTCCTTGTTGGTCTTT	100	50	236,660–236,677
1	C: AAAGACCAACAAGGAGTT	100	50	236,660–236,677
2	W: AGAAAACAGACTGTAGAT	50	90	238,359–238,376
2	C: ATCTACAGTCTGTTTTCT	50	90	238,359–238,376
3	W: ATATACATGTGGGTGCCT	90	70	240,627–240,644
3	C: AGGCACCCACATGTATAT	90	70	240,627–240,644
4	W: GAAAAGCCACAGGAGGTT	50	90	240,917–240,934
4	C: AACCTCCTGTGGCTTTTC	50	90	240,917–240,934
5	W: ATTAAAATGTTGTTTTAT	90	50	241,485–241,502
5	C: ATAAAACAACATTTTAAT	90	50	241,485–241,502
6	W: ATTCTCATGCAAGTACAT	90	60	241,527–241,544
6	C: ATGTACTTGCATGAGAAT	90	60	241,527–241,544
7	W: GGATAACAGCCTGTAAAA	50	90	243,048–243,065
7	C: TTTTACAGGCTGTTATCC	50	90	243,048–243,065
8	W: ACTGACAAGTAGTGGTGT	90	50	245,924–245,941
8	C: ACACCACTACTTGTCAGT	90	50	245,924–245,941
Total number of predicted topo II sites		8
Total length of predicted topo II sites		144 bp
Length of the AF9 SAR region		10,234 bp
Overall content of topo II sites		1.41%
AF9 non-SAR region (nucleotide position: 71116–75,277)
1	W: ATCAACATTCAGTTGTAT	90	50	73,170–73,187
1	C: ATACAACTGAATGTTGAT	90	50	73,170–73,187
Total number of predicted topo II sites		1
Total length of predicted topo II sites		18 bp
Length of the AF9 non-SAR region		4162 bp
Overall content of topo II sites		0.43%

ISSN: 1755-8794