Fig. 2

Effect of budesonide and dexamethasone on gene expression in A549 cells. A549 cells were either not treated or treated with 300 nM budesonide (Bud) or 1 μM dexamethasone (Dex). After 6 h, RNA was prepared, and microarray analysis performed using Affymetrix Primeview arrays. Probe set intensities (N = 3) from the different samples were normalized using the robust multi-array average (RMA) method and used to calculate the fold change compared to untreated (Additional file 4). a. Fold change, compared to untreated, for the dexamethasone and budesonide treatments, is plotted for each gene on the x and y axes, respectively. b. The relationship between the fold change for each treatment is shown in respect of all genes induced ≥2 fold (P ≤ 0.05) by either budesonide or dexamethasone treatment. Genes selected for qPCR validation are shown as circles (○) labelled with their gene symbol. c. A549 cells were either not treated or treated with 300 nM budesonide or 1 μM dexamethasone. After 6 h, RNA was extracted, and qPCR performed for the indicated genes and GAPDH. Data (N = 6), normalized to GAPDH, were expressed as fold relative to untreated control and are plotted as means ± SE