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Fig. 5 | BMC Medical Genomics

Fig. 5

From: Glucocorticoid-driven transcriptomes in human airway epithelial cells: commonalities, differences and functional insight from cell lines and primary cells

Fig. 5

Genes repressed by budesonide in A549, BEAS-2B and primary HBE cells. As detailed for Fig. 3, microarray analysis identified 425 genes as significantly repressed by budesonide (≤0.5 fold, P ≤ 0.05) in any of the three cell variants (Additional file 9). a. Genes meeting these criteria were used to produce a Venn diagram to illustrate commonality between the three cell variants. The 8 genes repressed in common are listed and, following analysis in DAVID, GO terms showing significant enrichment (EASE score ≤ 0.1) with the 8 genes from this list are shown. Genes representing each GO term are indicated. Terms are categorized into 4 functional groups, as indicated. b. Genes within each group (A = A549, B = BEAS-2B and H = HBE), according to the Venn diagram in a, are summarized by log2 intensity. Data are color-coded (Red = A549, yellow/orange = BEAS-2B, Blue = HBE) where open bars represent untreated (NS) and solid bars are budesonide (Bud) treatments. The box defines the upper and lower quartiles, and the line inside represents the median. Whiskers represent the 5th and 95th percentiles. c. The 425 genes were then grouped using the lower stringency, ≤0.8 fold, cut-off (Additional file 9). Heat maps were constructed for each of the groups. Data are presented as zero-mean log2 intensity, centered around the mean of untreated (NS) and treated for each gene in each cell variant. GO terms, showing enrichment (EASE score ≤ 0.1) and represented by at least 5 genes in the 82 gene repressed in common group, are listed. The number of genes associated with each term are indicated. Terms were then categorized into functional groups, as indicated

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