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Table 2 Linear positioning of centromeres of chromosomes: 4, 7, 8, 9, 10, 11, 18, X and Y in spermatozoa of 13 RCT carriers

From: Chromosome (re)positioning in spermatozoa of fathers and sons – carriers of reciprocal chromosome translocation (RCT)

Chromosome a - near the acrosome; m - middle; t - near the tail T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 T11 T12 T13 RCT mean value
± SD
[n = 13]
Control mean value
± SD
[n = 8]
t(1;11) t(2;8) t(2;10) t(3;9) t(4;10) t(4;18) t(6;14) t(7;18) t(11;13) t(4;5) father t(4;5) son t(7;10) father t(7;10) son
4 a 22.94 18.27 17.87 19.57 17.31 20.95 35.16 29.03 19.77 44.86 43.33 26.48 25.20 25.12 ± 5.56 15.46 ± 2.32
m 50.15 62.50 53.99 60.14 63.46 58.785 48.35 41.94 54.65 41.12 48.00 55.73 57.22 52.38 ± 6.43 60.11 ± 3.83
t 26.91 19.23 28.14 20.29 19.23 20.27 16.48 29.03 25.58 14.02 8.67 17.79 17.59 22.50 ± 4.61 24.42 ± 3.88
p [T vs. C] 0.0632 0.0425* 0.4572 0.4084 0.4697 0.2613 < 0.0001* < 0.0001* 0.4163 < 0.0001* < 0.0001* 0.0068* 0.0167*   
p [f vs. s]   0.1141 0.9483   
7 a 36.24 25.2 17.93 33.77 32.33 23.57 34.04 20.51 24.82 27.46 21.29 24.63 26.32 27.22 ± 5.99 19.01 ± 2.94
m 49.35 56.91 69.66 48.34 47.37 58.57 53.19 64.96 60.28 56.56 57.03 56.72 57.24 56.60 ± 6.90 66.85 ± 1.67
t 14.41 17.89 12.41 17.88 20.30 17.86 12.77 14.53 14.89 15.98 21.68 18.66 16.45 16.19 ± 2.55 14.14 ± 2.36
p [T vs. C] < 0.0001* 0.1060 0.8224 0.0002* 0.0001* 0.2125 0.0006* 0.9128 0.2921 0.0614 0.0568 0.0982 0.1018   
  p [f vs. s]   0.1930 0.8146   
8 a 16.05 32.22 13.51 20.95 21.43 14.29 28.57 14.14 26.32 13.13 23.29 21.90 28.13 21.59 ± 6.57 16.57 ± 2.48
m 57.31 54.44 55.41 50.00 55.19 63.27 51.19 53.54 48.03 61.95 52.97 61.98 53.13 54.86 ± 4.65 59.21 ± 1.76
t 26.65 13.33 31.08 29.05 23.38 22.45 20.24 32.32 25.66 24.92 23.74 16.12 18.75 23.55 ± 6.11 24.22 ± 3.11
p [Tvs. C] 0.8517 < 0.0001* 0.2526 0.1692 0.4216 0.6972 0.0054* 0.1646 0.0129* 0.6501 0.1834 0.1026 0.0070*   
  p [f vs. s]   0.0495* 0.1996   
9 a 29.57 26.74 36.42 33.61 31.25 29.75 38.67 20.55 16.78 21.77 31.71 27.74 29.58 29.15 ± 6.35 22.20 ± 1.24
m 53.48 56.98 49.01 56.30 54.17 54.43 50.67 67.81 59.44 64.25 55.40 57.66 59.15 56.28 ± 5.04 66.77 ± 2.28
t 16.96 16.28 14.57 10.08 14.58 15.82 10.67 11.64 23.78 13.98 12.89 14.60 11.27 14.57 ± 3.87 11.03 ± 1.45
p [T vs. C] 0.0160* 0.0879 0.0006* 0.0225* 0.0272* 0.0313* 0.0003* 0.9173 0.0002* 0.6400 0.0423* 0.1510 0.1894   
p [f vs. s]   0.0992 0.5667   
10 a 29.82 28.72 22.30 22.73 23.61 37.06 45.45 12.34 21.79 18.75 28.12 22.22 23.13 26.29 ± 8.83 26.79 ± 2.16
m 55.50 56.38 54.68 66.23 58.33 55.24 36.36 71.72 58.97 61.46 56.7 59.48 58.13 57.37 ± 8.66 60.47 ± 2.41
t 14.68 14.89 23.02 11.04 18.06 7.69 18.18 13.10 19.23 19.79 15.18 18.30 18.75 16.09 ± 4.32 12.74 ± 1.75
p [T vs. C] 0.5926 0.6775 0.0082* 0.4989 0.2625 0.0409* < 0.0001* 0.0064* 0.1178 0.0422* 0.6810 0.1997 0.1804   
p [f vs. s]   0.0854 0.9618   
11 a 24.82 24.55 30.91 23.53 27.97 25.00 30.88 34.21 21.74 23.86 25.87 25.81 29.88 27.21 ± 3.83 23.01 ± 1.93
m 56.20 70.00 53.94 54.71 54.55 60.00 52.21 51.32 61.74 49.62 54.44 55.38 49.39 56.31 ± 5.77 58.9 ± 1.76
t 18.98 5.45 15.15 21.76 17.48 15.00 16.91 14.47 16.52 26.52 19.69 18.82 20.73 16.48 ± 4.34 18.1 ± 2.83
p [T vs. C] 0.8569 0.0040* 0.1644 0.5944 0.4937 0.6964 0.1712 0.0279* 0.8416 0.0669 0.6594 0.7484 0.1375   
p [f vs. s]   0.2258 0.4827   
18 a 35.86 22.73 25.71 24.83 23.94 24.44 39.49 22.89 21.83 23.58 28.03 15.75 36.81 26.75 ± 7.35 22.75 ± 0.94
m 46.21 54.55 56.43 57.93 47.18 57.78 47.13 55.42 62.68 60.16 51.51 58.90 48.47 53.88 ± 5.68 57.47 ± 1.55
t 17.93 22.73 17.86 17.24 28.87 17.78 13.38 21.69 15.49 16.26 20.46 25.34 14.72 19.37 ± 4.76 19.79 ± 1.43
p [T vs. C] 0.0070* 0.7464 0.7437 0.7701 0.0480* 0.8473 0.0003* 0.8797 0.4858 0.6751 0.3933 0.1536 0.0033*   
p [f vs. s]   0.2208 < 0.0001*   
X a 50.00 27.50 34.62 51.80 36.96 44.00 47.33 39.29 41.5 43.02 23.97 21.01 40.44 39.30 ± 9.30 40.77 ± 2.74
m 44.40 65.00 56.92 44.88 51.45 52.71 43.51 52.68 56.13 45.35 68.49 69.57 47.79 53.98 ± 8.29 53.16 ± 2.40
t 5.60 7.50 8.46 3.32 11.59 3.29 9.16 8.04 2.37 11.63 7.54 9.42 11.76 7.79 ± 3.28 6.07 ± 2.01
p [T vs. C] 0.1678 0.0261* 0.3439 0.0633 0.0662 0.4647 0.1119 0.7056 0.2961 0.0415* 0.0029* 0.0003* 0.0529   
p [f vs. s]   < 0.0001* < 0.0001*   
Y a 40.31 20.75 21.77 46.17 27.74 46.38 37.59 31.40 37.50 32.82 21.71 18.84 34.97 32.72 ± 9.79 32.48 ± 8.52
m 52.04 60.38 66.13 49.88 56.20 48.42 50.83 61.35 57.42 51.15 61.24 68.84 58.04 56.75 ± 6.65 56.87 ± 7.08
t 7.65 18.87 12.10 3.95 16.0645 5.20 11.58 7.25 5.08 16.03 17.05 12.32 6.99 10.53 ± 4.82 10.66 ± 3.92
p [T vs. C] 0.2072 0.0046* 0.0730 0.0044* 0.1795 0.0067* 0.4665 0.4772 0.1571 0.1936 0.0209* 0.0142* 0.4775   
p [f vs. s]   0.0246* 0.0012*   
  1. Frequency (%) of centromeres was estimated in three regions of sperm cell nucleus: ‘a’ – near the acrosome, ‘m’ – its middle part and ‘t’ – near the tail of spermatozoa (according to the scheme presented in Fig. 1a). All RCT results were compared to the mean Control value obtained for 8 healthy donors (p values: T vs. C). Additionally, comparison between fathers and sons in familial RCT cases was performed: 46,XY,t(4;5)(p15.1;p12) (T10 vs. T11) and 46,XY,t(7;10)(p21.1;q26.13) (T12 vs. T13) (p values: f vs. s). One hundred and-fifty of centromeres were counted for each male and per each chromosome. Values p < 0.05 indicated statistically significant differences (two-tailed χ2 test) and were marked with ‘*’ in the Table