Fig. 6

S100A genes are associated with trastuzumab resistance. a The levels of the indicated S100A genes in wild type SKBR3 (SKBR3 wt) and trastuzumab resistant cells, SKBR3/100–8 were examined by RT-qPCR. The bars indicated relative expressing levels of the indicated S100A genes adjusted for 18S. Each bar indicated mean of three values plus standard deviation. **P < 0.01 compared to the levels of the indicated S100A genes in SKBR3 wt. b SKBR3/100–8 cells were treated with 10 μM of A485 for 24 h and the levels of the indicated S100A genes were determined by RT-qPCR. The bars indicated relative expressing levels of the indicated S100A genes adjusted for 18S. Each bar indicated mean of three values plus standard deviation. **P < 0.01 compared non-treated cells. c SKBR3 cells were plated in 96 well plate and treated with siRNA S100A6 for 2 days, and then 20 μg of EGF were added into culture for additional 24 h. Cells viability of each condition was measured by MTT assay. The bars were mean of 6 values plus standard deviation. **P < 0.01 compared to no EGF and sRNA treatment. d SKBR3/100–8 cells were treated with or without siRNA S1006 for 72 h and then RNA was extracted. The levels of S100A and TWIST1 were examined by RT-qPCR. The bars indicated relative expressing levels of S100A6 and TWIST1 genes adjusted for 18S. Each bar indicated mean of three values plus standard deviation. **P < 0.01 compared non-siRNA treated cells (Scrambled)