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Fig. 4 | BMC Medical Genomics

Fig. 4

From: Design and analysis of stably integrated reporters for inducible transgene expression in human T cells and CAR NK-cell lines

Fig. 4

Real-time fluorescence microscopy analysis of activity of the activation-inducible promoter 10xNFkB in Jurkat T cells. (а) Structure of the lentiviral constructs used for transduction. 10xNFkB-sfGFP module is oriented antisense relative to the 5’LTR-3’LTR of the vector. (b) Mean cell fluorescence values are plotted for the 10xNFkB-bearing and promoterless (neg. control) construct in the CD3/CD28-activated transduced cells as a function of time. Dynamics of the fluorescence signal in unstimulated 10xNFkB Jurkat cells is not shown on the plot, as it was indistinguishable from the control

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BMC Medical Genomics

ISSN: 1755-8794

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