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Fig. 7 | BMC Medical Genomics

Fig. 7

From: Structural variation of centromeric endogenous retroviruses in human populations and their impact on cutaneous T-cell lymphoma, Sézary syndrome, and HIV infection

Fig. 7

Barcoding of Amplicons of K111 in CTCL patients. The diagram depicts the 5′ portion of K111 that was amplified with primers P1F and 2499R, or the 3’portion of K111 amplified with primers 6535F and P2R. DNA samples were bar-coded so that they could be identified when these PCR amplicons were used for deep sequencing using the PacBio technology as described in Materials and Methods. Strong bands between 3 Kb to 4 Kb were cut from gels and purified for deep-sequencing analysis. The −/− K111 genotype did not provide an amplicon with P1F and 2499R. Two samples that did not amplify using the primers 6355F and P2R had likely interference with the barcode used. Using a different barcode, these samples produced the right amplification products (data not shown)

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BMC Medical Genomics

ISSN: 1755-8794

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