DNA methylation screening after roux-en Y gastric bypass reveals the epigenetic signature stems from genes related to the surgery per se

Table 1 CpG sites which were differently methylated between obese patients before the Roux-en Y gastric bypass and normal weight women and changed with surgical procedure

TargetID	CHR	Gene name	Gene region	Gene context	Methylation level normal weight	Methylation level pre-surgery	Methylation level post-surgery	Δ*	p*	Δ**	p**
cg04789056	14	C14orf93;C14orf93;C14orf93	3’UTR;3’UTR;3’UTR	S_Shelf	0.90 ± 0.02	0.76 ± 0.03	0.89 ± 0.03	−0.13	0.0013	0.13	0.0032
cg01219670	7	ZP3;ZP3	Body;Body		0.79 ± 0.02	0.69 ± 0.07	0.76 ± 0.05	−0.10	0.0003	0.07	0.0009
cg05590156	5	TRIO	Body		0.81 ± 0.02	0.71 ± 0.07	0.77 ± 0.06	−0.10	0.0003	0.06	0.0020
cg02747563	7			N_Shelf	0.94 ± 0.02	0.87 ± 0.08	0.92 ± 0.08	−0.07	0.0093	0.05	0.0011
cg03030650	2	SPEG	Body	S_Shelf	0.95 ± 0.01	0.90 ± 0.02	0.95 ± 0.01	−0.05	0.0019	0.05	0.0046
cg04934776	1	C1orf189;C1orf43;C1orf43;C1orf43	TSS1500;3’UTR;3’UTR;3’UTR		0.88 ± 0.01	0.82 ± 0.02	0.88 ± 0.02	−0.05	0.0036	0.05	0.0004
cg03441544	15	CYFIP1;CYFIP1	Body;Body		0.93 ± 0.01	0.87 ± 0.02	0.93 ± 0.01	−0.05	0.0029	0.05	0.0014
cg02212698	14	FOXN3;FOXN3	3’UTR;3’UTR	N_Shore	0.96 ± 0.01	0.91 ± 0.01	0.97 ± 0.01	−0.05	0.0013	0.05	0.0002
cg05486872	18	MYOM1;MYOM1	3’UTR;3’UTR	N_Shore	0.33 ± 0.07	0.40 ± 0.08	0.47 ± 0.11	0.07	0.0080	0.08	0.0008

Values showed in mean ± standard deviation; CHR: chromosome; Δ*: variation between pre-surgery patients and normal weight women; Δ**: variation between pre-surgery and post-surgery patients; p*: comparing pre-surgery patients and normal weight women; p**: comparing pre-surgery and post-surgery patients; UTR: untranslated region; TSS: transcription start site; C14orf93: chromosome 14 open reading frame 93; ZP3: zona pellucida glycoprotein 3; TRIO: trio Rho guanine nucleotide exchange factor; SPEG: SPEG Complex Locus; C1orf189: chromosome 1 open reading frame 189; C1orf43: chromosome 1 open reading frame 43; CYFIP1: cytoplasmic FMR1 interacting protein 1; FOXN3: forkhead box N3; MYOM1: myomesin 1

ISSN: 1755-8794