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Fig. 1 | BMC Medical Genomics

Fig. 1

From: Transcriptional consequences of impaired immune cell responses induced by cystic fibrosis plasma characterized via dual RNA sequencing

Fig. 1

Illustration of the workflow. Healthy donor PBMCs, stimulated with plasma from 9 CF patients and 3 HC, were the source of RNA for transcriptome sequencing. The CF subjects were diagnosed based on CFTR genotype and sweat chloride test using published guidelines [36, 37]. Following culture, the extracted total RNA from each sample was processed for simultaneous total RNA-Seq and miRNA-Seq. RNA-Seq was performed using the Illumina Next-Seq instrument at the RNA-Seq Center, Division of Pulmonary and Critical Care, Northwestern University, while miRNA-Seq was performed in-house using an Illumina MiSeq instrument. The generated expression signatures were analyzed to identify CF-relevant molecules while patient biochemical, clinical, and genetic variables were utilized for statistical analyses

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