Fig. 1

CB660 NSCs and GliNS2 CSCs have distinct gene expression profiles. a, Heatmap showing the normalized gene expression of the most variably expressed genes used for hierarchical clustering analysis. The top dendrogram shows that CB660 NSCs and GliNS2 CSCs cluster into two distinct populations. In the row above the heatmap, blue indicates CB660 NSCs and red indicates GliNS2 CSCs. b, Principal component analysis (PCA) and cell cycle phase analysis of CB660 NSCs and GliNS2 CSCs. The graph shows the separation of CB660 NSCs (circles) and GliNS2 CSCs (triangles) into distinct groups. The color of the circles or triangles corresponds to the predicted cell cycle phase of each cell. c, Pie plots showing the fraction of CB660 NSCs and GliNS2 CSCs in each phase of the cell cycle. P > 0.05; Chi-square test comparing the cell cycle profiles of CB660 NSCs and GliNS2 CSCs