Fig. 6
From: DNA methylation status of TBX20 in patients with tetralogy of Fallot
The influence of TBX20_M1 methylation on Sp1 transcription factor binding. Lane 1 contains only the Bio-labelled probe (TBX20_-708ābp/āā684ābp) alone; Lane 2: Bio-labelled probe + Sp1 protein in HEK 293āT cells; Lane 3: Bio-labelled probe + Sp1 protein + unlabelled competitor Mut probe; Lane 4: Mut-Bio-labelled probe + Sp1 protein in HEK 293āT cells; Lane 5: Bio-labelled probe + Sp1 protein + unlabelled competitor WT probe; Lane 6: Bio-labelled probe + Sp1 protein + unlabelled competitor Met probe (10-fold); Lane 7: Bio-labelled probe + Sp1 protein + unlabelled competitor Met probe (100-fold)