Unbalanced segregation of a paternal t(9;11)(p24.3;p15.4) translocation causing familial Beckwith-Wiedemann syndrome: a case report

Table 1 PCR and sequencing primers^a for bisulfite pyrosequencing

Region	Primer	Sequence (5′ to 3′)	CpGs	Chromosomal localization (bp)^b	Amplicon size (bp)	Sequence to analyze
ICR1	Forward	TGGGTATTTTTGGAGGTTTTTTT	4	Chr11: 2,020,978 - 2,021,291	314	YGYGTYGTAGGGTTTAYGGGGGTT
	Reverse	^cAACTTAAATCCCAAACCATAACA
	Sequencing	GTTTYGGGTTATTTAAGTTA
ICR2	Forward	TTGTTTATAAGGTGTAGATGGGAG	4	Chr11: 2,720,465 - 2,720,669	205	AYGTTTGTGATTTGGGAYGGTYGYGGGGTATATAGTTTATT
	Reverse	^cTCTCCCAAACTCTCCTCAAC
	Sequencing	TAGGTTAGGTTGTATTGTTG

ISSN: 1755-8794