Fig. 4

Pathway analysis of differentially expressed genes. Genes differentially expressed between either the high BMI control group (HiC) or the low (LoG) or high (HiG) BMI gastroparesis groups and the low BMI control group (LoC) were analyzed in two different ways. In the first, log2 fold changes were calculated on all samples relative to the low BMI samples, the resultant gene sets were then filtered to remove genes located on the Y chromosome. In a second approach samples were compared only to other samples obtained from subjects of the same gender (Additional file 6 : Table S3). Differentially expressed genes were then filtered to those exhibiting a logFC of − 1 or less or of + 1 or greater with a FDR of < 0.05 (i.e. genes with a 2 or greater fold change in expression). A comparison analysis was then performed to identify those genes whose change in expression was significant no matter which analysis was performed. These lists of genes were then subject to Ingenuity pathway analysis. The resultant top 5 canonical pathways, upstream regulators and diseases are shown