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Fig. 5 | BMC Medical Genomics

Fig. 5

From: Transcriptome profiling reveals significant changes in the gastric muscularis externa with obesity that partially overlap those that occur with idiopathic gastroparesis

Fig. 5

Predicted PDGF BB regulated genes are downregulated in gastroparesis subjects. Consistent with the qRT-PCR data shown in Fig. 1b, Ingenuity pathway analysis revealed that PDGF BB signaling may be altered in subjects with gastroparesis (Fig. 4 and Additional file 7: Table S4). Total RNA was isolated from the muscle layer of biopsies obtained from 9 control subjects with low BMI, 10 control subjects with high BMI and 13 subjects with idiopathic gastroparesis and low BMI. qRT-PCR was used to verify some of these changes in PDGF BB dependent mRNAs. Data presented were normalized to an internal control encoding TBP and are expressed relative to levels in the control low BMI subjects. Relative expression =2-ΔΔCt, where ΔΔCt = (CtHigh BMI or Gastroparesis-CtTBP) – (CtLow BMI control-CtTBP). Each point represents an individual subject. The mean ± SEM are also indicated. Significant differences between groups were identified using one-way Anova analysis with a Kruskal-Wallis test and a Duns Multiple Comparison post-test

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