Fig. 1
From: Retroposed copies of RET gene: a somatically acquired event in medullary thyroid carcinoma
Chart obtained by Coffalyzer analysis showing relative probe ratio values from MLPA results of RET gene analysis. The upper and lower bars represent the threshold values of normality. MLPA shows normal ratio for RET exons 1–20 in (a) normal thyroid tissue (b) peripheral blood and (c) C-cell hyperplasia, corroborating normal copy numer. Bullets located upper the duplication cut-off line (upper bar) in the ratio chart were identified in (d) Medullary Thyroid Carcinoma (MTC) and MTC- derived cell lines (E) MZ-CRC-1 cell line and (f) TT cell line, which denoted somatic duplication