Table 1 Primers for realtime PCR
# | Gene | Forward Primer (5′ - > 3′) | Reverse Primer (5′ - > 3′) | ||
|---|---|---|---|---|---|
Sequence | -mer | Sequence | -mer | ||
1 | c-Kit | CCACACCCTGTTCACTCCTT | 20 | TTCTGGGAAACTCCCAT TTG | 20 |
2 | Oct-4 | CTTGCTGCAGAAGTGGGTGGAGGAA | 25 | CTGCAGTGTGGGTTTCGGGCA | 21 |
3 | Sox-2 | TTGCTGCCTCTTTAAGACTAGGA | 23 | CTGGGGCTCAAACTTCTCTC | 20 |
4 | c-Myc | TCAAGAGGCGAACACACAAC | 20 | GGCCTTTTCATTGTTTTCCA | 20 |
5 | Klf-4 | AAGCCAAAGAGGGGAAGACG | 20 | CATGTGTAAGGCGAGGTGGT | 20 |
6 | Vasa | CTTAGACCCAGACGAATGTATGC | 23 | GTTCACTTCCACTGCCACTTC | 21 |
7 | Boll | GCAAGAAGAGCCTTGTTAATG | 21 | CCTCAGAAGGTTGCAGGTATAAG | 23 |
8 | Stella | GCGGAGTTCGTACGCATGA | 19 | CCATCCATTAGACACGCAGAAA | 22 |
9 | Dazl | GCTCGCCTGACGCCATCTTTG | 21 | GCTGATGAGGACTGGGTGCTG | 21 |
10 | Piwil-2 | TGGTTGGAGTAGGACGCTTG | 20 | GGGACGGTGTGCTGAAGG | 18 |
11 | Fragilis | GCACCCTCTACCTGAATCTG | 20 | AGGATGTTGTAGCACTTGGC | 20 |
12 | Sycp-3 | TGCAGGAGTAGTTGAAGATATG | 22 | CTAGCATGTCCTTAAGAAGCCTGTC | 25 |
13 | Stra-8 | AAGGACAGCGGCGTGG AC | 18 | CTGGCAAGCACTGAACTGGAG | 21 |
14 | GAPDH (Reference gene) | ACCATCTTCCAGGAGCGAGA | 20 | AGTGATGGCATGGACTGTGG | 20 |